Four volunteers will set off on a 90-mile journey from Sheffield Arena to Belfast Odyssey tomorrow.
The walk aims to raise £20,000 for two ice hockey fans.
Half of the money will go to the Amy Usher Fund, set up in memory of Steelers fan Amy, who died of cancer in 2015, aged 21. The money raised in her name will go to Weston Park Cancer Hospital.
The second half of the money will go towards a £10,000 wheelchair for Belfast Giants fan Blake McCaughey, who suffers from a rare genetic disorder.
David Simms, 52, Elliott Hall, 58, Dean Woolley, 39, and Neil Edwards, 48, will leave from the Sheffield Arena, heading across the Pennines through Castleton, Stockport and onwards to Liverpool.
They will then board the ferry at Birkenhead to Belfast before an appearance at the Belfast Giants v Sheffield Steelers match on Saturday, at the Odyssey, before heading back.
Elliott Hall, one of the volunteers, said: “Sheffield Steelers have always been part of the community, and the volunteers are part of that club community spirit.
“We are just middle-aged old idiots who want to do something to help those suffering and their families.”
This is not the first walk the volunteers have done. Three years ago, the volunteers walked from Sheffield to Coventry for Amy Usher. The group raised £24,183.
You can donate to the Sheffield Steelers volunteers here: https://www.gofundme.com/simmsey-and-friends-again